Bio-Chemical Tests for the Detection of Proteins

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  Biuret Test for the detection of Proteins

  Warming test for the detection of Proteins:

The test solution is to be heated in a boiling water bath, then proteins get coagulated.

• Test with trichloroacetic acid for the detection of Proteins:

Add trichloroacetic acid to the test solution, precipitate is produced.

• Biuret test for the detection of Proteins:

To the 2 ml of test solution, add 2 ml of Biuret reagent, if violet colour is formed, it indicates the presence of proteins.

• Hydrolysis test for the detection of Proteins:

Hydrolyze the test solution with hydrochloric acid or sulphuric acid. Then Ninhydrine test for amino acids has to be carried out.

• Xanthoproteic test for the detection of Proteins:

To the 5 ml of test solution, add 1 ml of concentrated nitric acid and boil, yellow precipitate is formed. After cooling it, add 40% sodium hydroxide solution, orange colour is observed.